BAIT
UBE2I
C358B7.1, P18, UBC9, LA16c-358B7.1
ubiquitin-conjugating enzyme E2I
GO Process (8)
GO Function (9)
GO Component (6)
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- protein ubiquitination [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TCF3
E2A, E47, ITF1, TCF-3, VDIR, bHLHb21
transcription factor 3
GO Process (15)
GO Function (17)
GO Component (7)
Gene Ontology Biological Process
- B cell differentiation [NAS]
- B cell lineage commitment [IDA, NAS]
- immunoglobulin V(D)J recombination [IDA]
- muscle cell differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of B cell proliferation [IMP]
- positive regulation of cell cycle [IDA]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of neuron differentiation [ISS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- positive regulation of transcription, DNA-templated [IDA, ISS]
- regulation of G1/S transition of mitotic cell cycle [IDA]
- regulation of transcription, DNA-templated [NAS]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- DNA binding [IDA, NAS]
- E-box binding [IDA, ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- enhancer binding [IC]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI, NAS]
- protein homodimerization activity [IDA]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- vitamin D response element binding [IDA]
- DNA binding [IDA, NAS]
- E-box binding [IDA, ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- enhancer binding [IC]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI, NAS]
- protein homodimerization activity [IDA]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- vitamin D response element binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Characterization of the mUBC9-binding sites required for E2A protein degradation.
Mammalian Ubc9 (mUbc9) is required for rapid degradation of the E2A proteins E12 and E47 by the ubiquitin-proteasome system. We have shown elsewhere that mUbc9 interacts with amino acids 477-530 of E12/E47. Here we test the hypothesis that this region, rich in proline, glutamic acid, serine, and threonine (PEST) residues, serves as the E2A protein degradation domain (DD). An E2A ... [more]
J. Biol. Chem. Oct. 01, 1999; 274(40);28690-6 [Pubmed: 10497239]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2I TCF3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID