Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

SCAMP1 function in endocytosis.

Fernandez-Chacon R, Achiriloaie M, Janz R, Albanesi JP, Suedhof TC

Secretory carrier membrane proteins (SCAMPs) are ubiquitous components of recycling vesicles that shuttle between the plasma membrane, endosomes, and the trans-Golgi complex. SCAMPs contain multiple N-terminal NPF repeats and four highly conserved transmembrane regions. NPF repeats often interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. We now show ... [more]

J. Biol. Chem. Apr. 28, 2000; 275(17);12752-6 [Pubmed: 10777571]

Throughput

  • Low Throughput

Curated By

  • BioGRID