Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Guo X, Zhu R, Luo A, Zhou H, Ding F, Yang H, Liu Z

Overexpression of eukaryotic translation initiation factor 3H (EIF3H) predicts cancer progression and poor prognosis, but the mechanism underlying EIF3H as an oncogene remains unclear in esophageal squamous cell carcinoma (ESCC).TCGA database and the immunohistochemistry (IHC) staining of ESCC samples were used and determined the upregulation of EIF3H in ESCC. CCK8 assay, colony formation assay and transwell assay were performed to ... [more]

J Exp Clin Cancer Res Aug. 31, 2020; 39(1);175 [Pubmed: 32867821]

Throughput

  • High Throughput

Curated By

  • BioGRID