BAIT
AKAP1
AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17
A kinase (PRKA) anchor protein 1
GO Process (1)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
NME1
AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1
NME/NM23 nucleoside diphosphate kinase 1
GO Process (8)
GO Function (9)
GO Component (6)
Gene Ontology Biological Process
- DNA catabolic process [IDA]
- negative regulation of cell proliferation [TAS]
- nucleobase-containing small molecule interconversion [TAS]
- nucleobase-containing small molecule metabolic process [TAS]
- positive regulation of DNA binding [IDA]
- positive regulation of epithelial cell proliferation [IMP]
- regulation of apoptotic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 9.74 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID