BAIT
AKAP1
AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17
A kinase (PRKA) anchor protein 1
GO Process (1)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
PRKCQ
PRKCT, nPKC-theta, RP11-563J2.1
protein kinase C, theta
GO Process (25)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- T cell receptor signaling pathway [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- innate immune response [TAS]
- intracellular signal transduction [NAS]
- membrane protein ectodomain proteolysis [ISS]
- negative regulation of T cell apoptotic process [IMP]
- negative regulation of insulin receptor signaling pathway [IMP]
- phototransduction, visible light [TAS]
- platelet activation [TAS]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of T cell activation [ISS]
- positive regulation of T-helper 17 type immune response [ISS]
- positive regulation of T-helper 2 cell activation [ISS]
- positive regulation of interleukin-17 production [ISS]
- positive regulation of interleukin-4 production [ISS]
- protein ubiquitination [TAS]
- regulation of cell growth [NAS]
- regulation of platelet aggregation [ISS]
- regulation of rhodopsin mediated signaling pathway [TAS]
- regulation of transcription, DNA-templated [ISS]
- rhodopsin mediated signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 440.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID