BAIT
AKAP1
AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17
A kinase (PRKA) anchor protein 1
GO Process (1)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
NAMPT
1110035O14Rik, PBEF, PBEF1, VF, VISFATIN
nicotinamide phosphoribosyltransferase
GO Process (13)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- NAD metabolic process [TAS]
- adipose tissue development [IDA]
- cell-cell signaling [TAS]
- circadian regulation of gene expression [ISS]
- insulin receptor signaling pathway [IDA]
- nicotinamide metabolic process [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of nitric-oxide synthase biosynthetic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 8.75 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID