BAIT
ANKFY1
ANKHZN, BTBD23, ZFYVE14
ankyrin repeat and FYVE domain containing 1
GO Process (5)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DVL3
dishevelled segment polarity protein 3
GO Process (9)
GO Function (6)
GO Component (1)
Gene Ontology Biological Process
- Wnt signaling pathway [IDA]
- Wnt signaling pathway, planar cell polarity pathway [IDA]
- canonical Wnt signaling pathway [IDA, IMP]
- non-canonical Wnt signaling pathway [IMP]
- non-canonical Wnt signaling pathway via JNK cascade [ISS]
- planar cell polarity pathway involved in neural tube closure [IBA]
- positive regulation of JUN kinase activity [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 4.2 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID