BAIT
ARF6
ADP-ribosylation factor 6
GO Process (14)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- cell adhesion [TAS]
- cellular component movement [TAS]
- cortical actin cytoskeleton organization [IMP]
- negative regulation of receptor-mediated endocytosis [TAS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of establishment of protein localization to plasma membrane [ISS]
- protein localization to cell surface [ISS]
- protein localization to endosome [IMP]
- regulation of Rac protein signal transduction [IDA]
- regulation of dendritic spine development [ISS]
- regulation of filopodium assembly [IDA]
- ruffle organization [IDA]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PLCB1
EIEE12, PI-PLC, PLC-154, PLC-I, PLC154, PLCB1A, PLCB1B, RP4-654A7.1
phospholipase C, beta 1 (phosphoinositide-specific)
GO Process (29)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- G-protein coupled acetylcholine receptor signaling pathway [ISS]
- G2/M transition of mitotic cell cycle [ISS]
- activation of meiosis involved in egg activation [ISS]
- cerebral cortex development [ISS]
- glutamate receptor signaling pathway [ISS]
- inositol phosphate metabolic process [TAS]
- insulin-like growth factor receptor signaling pathway [ISS]
- interleukin-1-mediated signaling pathway [IDA]
- interleukin-12-mediated signaling pathway [IDA]
- interleukin-15-mediated signaling pathway [IDA]
- memory [ISS]
- negative regulation of monocyte extravasation [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- phosphatidylinositol metabolic process [ISS]
- positive regulation of CD24 biosynthetic process [ISS]
- positive regulation of G1/S transition of mitotic cell cycle [ISS]
- positive regulation of GTPase activity [IDA]
- positive regulation of JNK cascade [IDA]
- positive regulation of acrosome reaction [ISS]
- positive regulation of developmental growth [ISS]
- positive regulation of embryonic development [ISS]
- positive regulation of interleukin-12 production [ISS]
- positive regulation of myoblast differentiation [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of G-protein coupled receptor protein signaling pathway [ISS]
- regulation of fertilization [ISS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 145.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID