BAIT
ARF6
ADP-ribosylation factor 6
GO Process (14)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- cell adhesion [TAS]
- cellular component movement [TAS]
- cortical actin cytoskeleton organization [IMP]
- negative regulation of receptor-mediated endocytosis [TAS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of establishment of protein localization to plasma membrane [ISS]
- protein localization to cell surface [ISS]
- protein localization to endosome [IMP]
- regulation of Rac protein signal transduction [IDA]
- regulation of dendritic spine development [ISS]
- regulation of filopodium assembly [IDA]
- ruffle organization [IDA]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAP1B
K-REV, RAL1B, OK/SW-cl.11
RAP1B, member of RAS oncogene family
GO Process (10)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- Rap protein signal transduction [IMP]
- blood coagulation [TAS]
- cellular response to cAMP [IDA]
- energy reserve metabolic process [TAS]
- establishment of endothelial barrier [IMP]
- platelet activation [TAS]
- regulation of cell junction assembly [IMP]
- regulation of establishment of cell polarity [IMP]
- regulation of insulin secretion [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 35.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID