BAIT
C11ORF52
chromosome 11 open reading frame 52
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
DOCK7
EIEE23, ZIR2, RP4-740B20.2
dedicator of cytokinesis 7
GO Process (8)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- activation of Rac GTPase activity [IDA]
- axonogenesis [IMP]
- establishment of neuroblast polarity [IMP]
- interkinetic nuclear migration [ISS]
- microtubule cytoskeleton organization [IMP]
- neuron projection development [IMP]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- regulation of neurogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 8.33 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID