BAIT
C11ORF52
chromosome 11 open reading frame 52
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
SRGAP2
ARHGAP34, FNBP2, SRGAP2A, SRGAP3, RP11-312O7.2
SLIT-ROBO Rho GTPase activating protein 2
GO Process (14)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- actin filament severing [IDA]
- axon guidance [TAS]
- cell proliferation [IMP]
- dendritic spine development [IDA]
- extension of a leading process involved in cell motility in cerebral cortex radial glia guided migration [ISS]
- filopodium assembly [IDA]
- lamellipodium assembly involved in ameboidal cell migration [IMP]
- negative regulation of cell death [IMP]
- negative regulation of neuron migration [IDA]
- neuron projection morphogenesis [ISS]
- positive regulation of Rac GTPase activity [IDA, IMP]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 5.43 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID