BAIT

RNF19A

RNF19
ring finger protein 19A, RBR E3 ubiquitin protein ligase
UPS Project
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

UBE2L6

RIG-B, UBCH8
ubiquitin-conjugating enzyme E2L 6
UPS Project
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity.

Niwa J, Ishigaki S, Doyu M, Suzuki T, Tanaka K, Sobue G

We cloned a novel gene, Dorfin (double ring-finger protein), from human spinal cord. The Dorfin mRNA transcript was 4.4 kb and expressed ubiquitously in many organs as well as in the central nervous system, including the spinal cord. Dorfin encoded 838 amino acid protein Dorfin, which contains two RING-finger motifs and an IBR (in between RING-fingers) motif at its N-terminus. ... [more]

Biochem. Biophys. Res. Commun. Mar. 02, 2001; 281(3);706-13 [Pubmed: 11237715]

Throughput

  • Low Throughput

Curated By

  • BioGRID