BAIT
CENPA
CENP-A, CenH3
centromere protein A
GO Process (6)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KMT2A
ALL-1, CXXC7, HRX, HTRX1, MLL, MLL/GAS7, MLL1, MLL1A, TET1-MLL, TRX1, WDSTS, hCG_1732268
lysine (K)-specific methyltransferase 2A
GO Process (14)
GO Function (11)
GO Component (5)
Gene Ontology Biological Process
- circadian regulation of gene expression [ISS]
- embryonic hemopoiesis [TAS]
- histone H3-K4 methylation [IDA, IMP]
- histone H3-K4 trimethylation [IDA]
- histone H4-K16 acetylation [IMP]
- positive regulation of cellular response to drug [IMP]
- positive regulation of histone H3-K4 methylation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of transporter activity [IMP]
- protein complex assembly [IDA]
- regulation of histone H3-K14 acetylation [ISS]
- regulation of histone H3-K9 acetylation [ISS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- AT DNA binding [NAS]
- core promoter sequence-specific DNA binding [ISS]
- histone methyltransferase activity (H3-K4 specific) [IDA, IMP]
- identical protein binding [IPI]
- lysine-acetylated histone binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
- unmethylated CpG binding [IDA]
- zinc ion binding [IDA]
- AT DNA binding [NAS]
- core promoter sequence-specific DNA binding [ISS]
- histone methyltransferase activity (H3-K4 specific) [IDA, IMP]
- identical protein binding [IPI]
- lysine-acetylated histone binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
- unmethylated CpG binding [IDA]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 10.18 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID