BAIT
CLTA
LCA
clathrin, light chain A
GO Process (7)
GO Function (2)
GO Component (9)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- axon guidance [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- membrane organization [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- post-Golgi vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
WNK3
PRKWNK3, RP3-390O13.2
WNK lysine deficient protein kinase 3
GO Process (13)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- intracellular signal transduction [ISS]
- negative regulation of apoptotic process [IMP]
- positive regulation of calcium ion transport [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of ion transmembrane transporter activity [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of rubidium ion transmembrane transporter activity [IDA]
- positive regulation of rubidium ion transport [IDA]
- positive regulation of sodium ion transmembrane transporter activity [IDA, IMP]
- positive regulation of sodium ion transport [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [ISS]
- regulation of ion homeostasis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 260.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID