BAIT
COIL
CLN80, p80-coilin
coilin
GO Process (0)
GO Function (3)
GO Component (6)
Gene Ontology Molecular Function
Homo sapiens
PREY
PFKL
ATP-PFK, PFK-B, PFK-L
phosphofructokinase, liver
GO Process (9)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- carbohydrate phosphorylation [IDA, IMP]
- fructose 1,6-bisphosphate metabolic process [IDA]
- fructose 6-phosphate metabolic process [IDA, IMP]
- glucose metabolic process [TAS]
- glycolytic process [IDA, TAS]
- protein oligomerization [IDA]
- response to glucose [IDA]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 5.47 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID