CS
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPIF
Gene Ontology Biological Process
- cellular response to arsenic-containing substance [ISS]
- cellular response to calcium ion [ISS]
- cellular response to hydrogen peroxide [IMP]
- negative regulation of ATPase activity [ISS]
- negative regulation of apoptotic process [IDA]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- negative regulation of oxidative phosphorylation [ISS]
- negative regulation of oxidative phosphorylation uncoupler activity [ISS]
- negative regulation of release of cytochrome c from mitochondria [IDA]
- positive regulation of release of cytochrome c from mitochondria [ISS]
- protein peptidyl-prolyl isomerization [IBA]
- regulation of mitochondrial membrane permeability [ISS]
- regulation of mitochondrial membrane permeability involved in programmed necrotic cell death [IMP]
- regulation of proton-transporting ATPase activity, rotational mechanism [ISS]
- response to ischemia [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Quantitative Score
- 165.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
CS PPIF | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 1 | BioGRID | 2850052 |
Curated By
- BioGRID