NCL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
XRN1
Gene Ontology Biological Process
- nonfunctional rRNA decay [IMP]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- positive regulation of transcription initiation from RNA polymerase II promoter [IMP]
- traversing start control point of mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.
Mature tRNA is normally extensively modified and extremely stable. Recent evidence suggests that hypomodified mature tRNA in yeast can undergo a quality control check by a rapid tRNA decay (RTD) pathway, since mature tRNA(Val(AAC)) lacking 7-methylguanosine and 5-methylcytidine is rapidly degraded and deacylated at 37 degrees C in a trm8-Delta trm4-Delta strain, resulting in temperature-sensitive growth. We show here that ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: protein/peptide accumulation (APO:0000149)
- phenotype: protein/peptide modification (APO:0000131)
Additional Notes
- tRNA Val(AAC) is aminoacylated to nearly WT levels and is not degraded in a trm8 trm4 rat1 xrn1 quadruple mutant.
- tRNAVal(AAC) is aminoacylated to nearly WT levels and is not degraded in a trm8 trm4 rat1 xrn1 quadruple mutant.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
NCL1 XRN1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1561 | BioGRID | 2077706 |
Curated By
- BioGRID