An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Insulin receptor substrate 4 associates with the protein IRAS.

Sano H, Liu SC, Lane WS, Piletz JE, Lienhard GE

The insulin receptor substrates (IRSs) are key components in signaling from the insulin receptor, and consequently any proteins that interact with them are expected to participate in insulin signaling. In this study we have searched for proteins that interact with IRS-4 by identifying the proteins that coimmunoprecipitated with IRS-4 from human embryonic kidney 293 cells by microsequencing through mass spectrometry. ... [more]

J. Biol. Chem. May. 31, 2002; 277(22);19439-47 [Pubmed: 11912194]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

IRS4

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3/SH2 adaptor activity [TAS]insulin receptor binding [IBA]phosphatidylinositol 3-kinase binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4