BAIT

NISCH

I-1, IR1, IRAS, hIRAS
nischarin
GO Process (0)
GO Function (2)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

IRS1

HIRS-1
insulin receptor substrate 1
GO Process (25)
GO Function (7)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Insulin receptor substrate 4 associates with the protein IRAS.

Sano H, Liu SC, Lane WS, Piletz JE, Lienhard GE

The insulin receptor substrates (IRSs) are key components in signaling from the insulin receptor, and consequently any proteins that interact with them are expected to participate in insulin signaling. In this study we have searched for proteins that interact with IRS-4 by identifying the proteins that coimmunoprecipitated with IRS-4 from human embryonic kidney 293 cells by microsequencing through mass spectrometry. ... [more]

J. Biol. Chem. May. 31, 2002; 277(22);19439-47 [Pubmed: 11912194]

Throughput

  • Low Throughput

Curated By

  • BioGRID