pumilio RNA-binding family member 2
GO Process (0)
GO Function (2)
GO Component (4)
Homo sapiens


NOS1, SPGF12, RP11-498J9.3
nanos homolog 1 (Drosophila)
GO Process (3)
GO Function (2)
GO Component (2)
Homo sapiens


Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.

Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA

Germ cells are the cells which ultimately give rise to mature sperm and eggs. In model organisms such as flies and worms, several genes that are required for formation and maintenance of germ cells have been identified and their interactions are rapidly being delineated. By contrast, little is known of the genes required for development of human germ cells and ... [more]

Dev. Genes Evol. Apr. 01, 2003; 213(3);120-6 [Pubmed: 12690449]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


Curated By

  • BioGRID