KRAS
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein phosphorylation [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAMP7
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [ISS]
- autophagic vacuole fusion [IMP]
- calcium ion-dependent exocytosis [ISS]
- endocytosis [IBA]
- endosome to lysosome transport [IDA]
- eosinophil degranulation [IMP, ISS]
- exocytosis [IBA]
- membrane organization [TAS]
- natural killer cell degranulation [IMP]
- neutrophil degranulation [IMP, ISS]
- phagocytosis, engulfment [ISS]
- positive regulation of histamine secretion by mast cell [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- vesicle fusion [IDA]
- vesicle-mediated transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- SNARE complex [IDA, ISS]
- azurophil granule membrane [IDA]
- cytoplasm [IDA]
- endoplasmic reticulum membrane [ISS]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- lamellipodium [IDA]
- late endosome membrane [ISS]
- lysosomal membrane [IDA, ISS, TAS]
- membrane [IDA]
- neuron projection [ISS]
- phagocytic vesicle [ISS]
- plasma membrane [IDA, TAS]
- platelet alpha granule [IDA]
- pseudopodium [IDA]
- secretory granule [IDA]
- secretory granule membrane [IDA]
- trans-Golgi network [IDA]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Quantitative Score
- 30.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KRAS VAMP7 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 7.0154 | BioGRID | 2604707 |
Curated By
- BioGRID