BAIT
LAMP2
CD107b, LAMP-2, LAMPB, LGP110
lysosomal-associated membrane protein 2
GO Process (4)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB13
GIG4
RAB13, member RAS oncogene family
GO Process (15)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISS]
- cortical actin cytoskeleton organization [IBA, ISS]
- endocytic recycling [IMP]
- endosomal transport [IMP]
- endothelial cell chemotaxis [ISS]
- establishment of Sertoli cell barrier [ISS]
- establishment of protein localization to plasma membrane [IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- neuron projection development [IMP]
- protein kinase A signaling [IMP]
- tight junction assembly [IMP]
- trans-Golgi network to recycling endosome transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- endocytic vesicle [IDA]
- extracellular vesicular exosome [IDA]
- insulin-responsive compartment [ISS]
- lamellipodium [ISS]
- lateral plasma membrane [IDA]
- neuron projection [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tight junction [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 65.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID