BAIT
LRRC59
p34, PRO1855
leucine rich repeat containing 59
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ENPP1
ARHR2, COLED, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1
ectonucleotide pyrophosphatase/phosphodiesterase 1
GO Process (21)
GO Function (10)
GO Component (7)
Gene Ontology Biological Process
- 3'-phosphoadenosine 5'-phosphosulfate metabolic process [IDA]
- ATP catabolic process [ISS]
- cellular phosphate ion homeostasis [IDA]
- cellular response to insulin stimulus [IDA]
- generation of precursor metabolites and energy [IDA]
- inorganic diphosphate transport [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of fat cell differentiation [IDA]
- negative regulation of glucose import [IDA]
- negative regulation of glycogen biosynthetic process [IDA]
- negative regulation of insulin receptor signaling pathway [IDA]
- negative regulation of protein autophosphorylation [IDA]
- nucleic acid phosphodiester bond hydrolysis [ISS]
- nucleoside triphosphate catabolic process [IDA]
- phosphate-containing compound metabolic process [IDA]
- regulation of bone mineralization [IC]
- riboflavin metabolic process [TAS]
- sequestering of triglyceride [IDA]
- small molecule metabolic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function- 3'-phosphoadenosine 5'-phosphosulfate binding [IC]
- ATP binding [IDA]
- calcium ion binding [ISS]
- insulin receptor binding [IDA]
- nucleoside-triphosphate diphosphatase activity [IDA]
- nucleotide diphosphatase activity [IDA, TAS]
- phosphodiesterase I activity [ISS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [ISS]
- 3'-phosphoadenosine 5'-phosphosulfate binding [IC]
- ATP binding [IDA]
- calcium ion binding [ISS]
- insulin receptor binding [IDA]
- nucleoside-triphosphate diphosphatase activity [IDA]
- nucleotide diphosphatase activity [IDA, TAS]
- phosphodiesterase I activity [ISS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 30.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID