BAIT
PARP1
ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, RP11-125A15.2
poly (ADP-ribose) polymerase 1
GO Process (12)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ERCC4
ERCC11, FANCQ, RAD1, XPF
excision repair cross-complementation group 4
GO Process (15)
GO Function (8)
GO Component (6)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IBA, IDA]
- DNA repair [IMP, TAS]
- double-strand break repair via homologous recombination [IMP]
- negative regulation of telomere maintenance [IMP]
- nucleotide-excision repair [IDA, IMP, TAS]
- nucleotide-excision repair involved in interstrand cross-link repair [IBA]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA incision [IDA]
- nucleotide-excision repair, DNA incision, 3'-to lesion [IMP]
- nucleotide-excision repair, DNA incision, 5'-to lesion [IMP]
- resolution of meiotic recombination intermediates [IBA]
- response to UV [IMP]
- telomere maintenance [IMP]
- telomere maintenance via telomere shortening [IMP]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 20.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID