BAIT
PARP1
ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, RP11-125A15.2
poly (ADP-ribose) polymerase 1
GO Process (12)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NIPBL
CDLS, CDLS1, IDN3, IDN3-B, Scc2
Nipped-B homolog (Drosophila)
GO Process (28)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- brain development [IMP]
- cellular protein localization [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to X-ray [IMP]
- cognition [IMP]
- developmental growth [IMP]
- ear morphogenesis [IMP]
- embryonic digestive tract morphogenesis [IMP]
- embryonic forelimb morphogenesis [IMP]
- external genitalia morphogenesis [IMP]
- eye morphogenesis [IMP]
- face morphogenesis [IMP]
- forelimb morphogenesis [IMP]
- gall bladder development [IMP]
- heart morphogenesis [IMP]
- maintenance of mitotic sister chromatid cohesion [IMP]
- metanephros development [NAS]
- mitotic cell cycle [TAS]
- mitotic sister chromatid cohesion [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [IMP]
- positive regulation of histone deacetylation [IDA]
- regulation of developmental growth [IMP]
- regulation of embryonic development [IMP]
- regulation of hair cycle [IMP]
- sensory perception of sound [IMP]
- uterus morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 8.03 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID