BAIT
PDHA1
PDHA, PDHAD, PDHCE1A, PHE1A, RP11-723P2.1
pyruvate dehydrogenase (lipoamide) alpha 1
GO Process (6)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SUPV3L1
SUV3, RP11-227H15.2
suppressor of var1, 3-like 1 (S. cerevisiae)
GO Process (14)
GO Function (10)
GO Component (5)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA duplex unwinding [IMP]
- DNA recombination [IMP]
- RNA catabolic process [IMP]
- chromatin maintenance [IMP]
- mitochondrial RNA 3'-end processing [IMP]
- mitochondrial RNA surveillance [IMP]
- mitochondrial mRNA catabolic process [IMP]
- mitochondrial mRNA surveillance [IMP]
- mitochondrial ncRNA surveillance [IMP]
- mitochondrion morphogenesis [IMP]
- negative regulation of apoptotic process [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of mitochondrial RNA catabolic process [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 165.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID