BAIT
PEX14
NAPP2, PBD13A, Pex14p, dJ734G22.2
peroxisomal biogenesis factor 14
GO Process (12)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- microtubule anchoring [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of protein homotetramerization [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- peroxisome organization [IGI, ISS]
- peroxisome transport along microtubule [IDA]
- protein complex assembly [IDA]
- protein homooligomerization [IDA]
- protein import into peroxisome matrix [IMP]
- protein import into peroxisome matrix, substrate release [IDA]
- protein import into peroxisome matrix, translocation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LMTK2
AATYK2, BREK, KPI-2, KPI2, LMR2, PPP1R100, cprk
lemur tyrosine kinase 2
GO Process (9)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- early endosome to late endosome transport [IMP]
- endocytic recycling [IMP]
- negative regulation of catalytic activity [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- receptor recycling [IMP]
- transferrin transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 90.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID