BAIT
RAB2A
LHX, RAB2
RAB2A, member RAS oncogene family
GO Process (6)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ITGA4
CD49D, IA4
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
GO Process (14)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- B cell differentiation [IC]
- blood coagulation [TAS]
- cell-matrix adhesion [IMP]
- cell-matrix adhesion involved in ameboidal cell migration [IMP]
- endodermal cell differentiation [IEP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- leukocyte tethering or rolling [IMP]
- negative regulation of protein homodimerization activity [IDA]
- receptor clustering [IMP]
- regulation of immune response [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 30.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID