BAIT
RAB2A
LHX, RAB2
RAB2A, member RAS oncogene family
GO Process (6)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SLC9A1
APNH, NHE-1, NHE1, PPP1R143, RP4-633N17.1
solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
GO Process (32)
GO Function (9)
GO Component (11)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cell migration [TAS]
- cellular response to acidic pH [IDA, ISS]
- cellular response to epinephrine stimulus [IMP]
- cellular response to mechanical stimulus [TAS]
- cellular sodium ion homeostasis [IDA]
- glycosaminoglycan metabolic process [TAS]
- hyaluronan catabolic process [TAS]
- hyaluronan metabolic process [TAS]
- hydrogen ion transmembrane transport [IDA]
- ion transport [TAS]
- maintenance of cell polarity [TAS]
- positive regulation of NFAT protein import into nucleus [IDA]
- positive regulation of calcineurin-NFAT signaling cascade [IDA]
- positive regulation of calcium:sodium antiporter activity [IMP]
- positive regulation of cardiac muscle hypertrophy [IMP]
- positive regulation of the force of heart contraction [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein oligomerization [ISS]
- regulation of cardiac muscle cell membrane potential [TAS]
- regulation of cardiac muscle contraction by calcium ion signaling [IMP]
- regulation of focal adhesion assembly [TAS]
- regulation of intracellular pH [IDA]
- regulation of pH [IDA, TAS]
- regulation of stress fiber assembly [TAS]
- regulation of the force of heart contraction by cardiac conduction [IMP]
- response to acidic pH [IDA]
- response to muscle stretch [IMP]
- small molecule metabolic process [TAS]
- sodium ion export [ISS]
- sodium ion import across plasma membrane [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function- calcium-dependent protein binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [TAS]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein complex scaffold [TAS]
- protein phosphatase 2B binding [IDA, IPI]
- sodium:proton antiporter activity [IDA, ISS]
- sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential [TAS]
- solute:proton antiporter activity [TAS]
- calcium-dependent protein binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [TAS]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein complex scaffold [TAS]
- protein phosphatase 2B binding [IDA, IPI]
- sodium:proton antiporter activity [IDA, ISS]
- sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential [TAS]
- solute:proton antiporter activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 50.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID