BAIT
RAB3B
RAB3B, member RAS oncogene family
GO Process (11)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- GTP catabolic process [IBA, IDA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- intracellular protein transport [IBA]
- positive regulation of dopamine uptake involved in synaptic transmission [IDA]
- protein localization to plasma membrane [IBA]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- regulation of vesicle size [IDA]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
VPS45
H1, H1VPS45, SCN5, VPS45A, VPS45B, VPS54A, VSP45, VSP45A, RP11-458I7.2
vacuolar protein sorting 45 homolog (S. cerevisiae)
GO Process (2)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 40.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID