BAIT
PUB1
SPAC11G7.02
HECT-type ubiquitin-protein ligase E3 Pub1
GO Process (7)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- negative regulation of leucine import in response to ammonium ion [IMP]
- protein localization to Golgi apparatus [IMP]
- protein ubiquitination [IMP]
- regulation of intracellular pH [IGI]
- regulation of mitotic cell cycle [IGI]
- regulation of transmembrane transport [IMP]
- ubiquitin-dependent protein catabolic process [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
CDC25
sal2, SPAC24H6.05
M phase inducer tyrosine phosphatase Cdc25
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- mitotic DNA replication checkpoint [IMP]
- peptidyl-tyrosine dephosphorylation involved in activation of protein kinase activity [IDA]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle [IMP]
- regulation of G2/M transition of mitotic cell cycle [IMP]
- regulation of cell size [NAS]
- signal transduction involved in intra-S DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Schizosaccharomyces pombe (972h)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Pub1 acts as an E6-AP-like protein ubiquitiin ligase in the degradation of cdc25.
The level of the mitotic activating tyrosine phosphatase cdc25 is regulated by both transcriptional and post-transcriptional mechanisms in the fission yeast Schizosaccharomyces pombe. We have found that cdc25 is ubiquitinated and have cloned pub1, a gene which regulates this event. Pub1 contains a region highly homologous to the putative catalytic domain of the human protein ubiquitin ligase E6-AP. Disruption of ... [more]
EMBO J. Mar. 15, 1996; 15(6);1301-12 [Pubmed: 8635463]
Throughput
- Low Throughput
Ontology Terms
- phenotype: temperature sensitive growth (APO:0000092)
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID