An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A proximity-dependent biotinylation map of a human cell.

Go CD, Knight JDR, Rajasekharan A, Rathod B, Hesketh GG, Abe KT, Youn JY, Samavarchi-Tehrani P, Zhang H, Zhu LY, Popiel E, Lambert JP, Coyaud E, Cheung SWT, Rajendran D, Wong CJ, Antonicka H, Pelletier L, Palazzo AF, Shoubridge EA, Raught B, Gingras AC

Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]

Nature Jun. 02, 2021; (); [Pubmed: 34079125]

Quantitative Score

32.53 [FoldChange]

Throughput

High Throughput

Additional Notes

BioID
SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RABEP1 RAB4A	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Vitale G (1998)	Low	-	BioGRID	-
RAB4A RABEP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Wilson B (2023)	High	-	BioGRID	3558220
RAB4A RABEP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Christoforidis S (2000)	Low	-	BioGRID	-
RAB4A RABEP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kaelin S (2015)	Low	-	BioGRID	-
RAB4A RABEP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Vitale G (1998)	Low	-	BioGRID	-
RABEP1 RAB4A	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2695700
RABEP1 RAB4A	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vitale G (1998)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAB4A

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP binding [IDA]GTP binding [IDA]GTPase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

RABEP1