BAIT
REEP5
C5orf18, D5S346, DP1, TB2, YOP1
receptor accessory protein 5
GO Process (0)
GO Function (1)
GO Component (1)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EIF2AK3
PEK, PERK, WRS
eukaryotic translation initiation factor 2-alpha kinase 3
GO Process (26)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- ER overload response [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- angiogenesis [IMP]
- bone mineralization [ISS]
- calcium-mediated signaling [ISS]
- cellular protein metabolic process [TAS]
- cellular response to glucose starvation [IMP]
- chondrocyte development [ISS]
- endocrine pancreas development [IMP]
- endoplasmic reticulum organization [ISS]
- endoplasmic reticulum unfolded protein response [IDA, TAS]
- insulin secretion [ISS]
- insulin-like growth factor receptor signaling pathway [ISS]
- negative regulation of myelination [ISS]
- negative regulation of translation [TAS]
- negative regulation of translational initiation in response to stress [TAS]
- ossification [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of transcription from RNA polymerase I promoter [IMP]
- positive regulation vascular endothelial growth factor production [IMP]
- protein autophosphorylation [IDA, IMP]
- protein homooligomerization [IMP]
- protein phosphorylation [ISS]
- response to endoplasmic reticulum stress [IMP]
- skeletal system development [ISS]
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 100.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID