Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Nesprin-1alpha self-associates and binds directly to emerin and lamin A in vitro.

Mislow JM, Holaska JM, Kim MS, Lee KK, Segura-Totten M, Wilson KL, McNally EM

Nesprin-1alpha is a spectrin repeat (SR)-containing, transmembrane protein of the inner nuclear membrane, and is highly expressed in muscle cells. A yeast two-hybrid screen for nesprin-1alpha-interacting proteins showed that nesprin-1alpha interacted with itself. Blot overlay experiments revealed that nesprin-1alpha's third SR binds the fifth SR. The carboxy-terminal half of nesprin-1alpha directly bound lamin A, a nuclear intermediate filament protein. Biochemical ... [more]

FEBS Lett. Aug. 14, 2002; 525(1);135-40 [Pubmed: 12163176]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SYNE1 SYNE1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Mislow JM (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SYNE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]actin filament binding [ISS]lamin binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [ISS]

Gene Ontology Cellular Component