Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Members of the Zyxin family of LIM proteins interact with members of the p130Cas family of signal transducers.

Yi J, Kloeker S, Jensen CC, Bockholt S, Honda H, Hirai H, Beckerle MC

Integrin binding to extracellular matrix proteins induces formation of signaling complexes at focal adhesions. Zyxin co-localizes with integrins at sites of cell-substratum adhesion and is postulated to serve as a docking site for the assembly of multimeric protein complexes involved in regulating cell motility. Recently, we identified a new member of the zyxin family called TRIP6. TRIP6 is localized at ... [more]

J. Biol. Chem. Mar. 15, 2002; 277(11);9580-9 [Pubmed: 11782456]

Throughput

  • Low Throughput

Curated By

  • BioGRID