BAIT
STX7
RP11-560I21.1
syntaxin 7
GO Process (8)
GO Function (4)
GO Component (18)
Gene Ontology Biological Process
- intracellular protein transport [IBA]
- organelle assembly [IDA]
- organelle localization [IDA]
- positive regulation of T cell mediated cytotoxicity [IMP]
- positive regulation of receptor localization to synapse [IMP]
- regulation of protein localization to plasma membrane [IDA]
- vesicle docking [IBA]
- vesicle fusion [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IBA]
- azurophil granule [IDA]
- early endosome [IDA]
- endocytic vesicle [IDA]
- endomembrane system [IBA]
- endosome [IDA]
- extracellular vesicular exosome [IDA]
- immunological synapse [IDA]
- integral component of membrane [IBA]
- intracellular membrane-bounded organelle [IDA]
- late endosome [IDA]
- lysosomal membrane [IDA]
- lysosome [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tertiary granule [IDA]
- vesicle [IDA]
Homo sapiens
PREY
MYO6
DFNA22, DFNB37, RP3-472A9.1
myosin VI
GO Process (9)
GO Function (7)
GO Component (19)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator [IDA]
- actin filament-based movement [ISS, NAS]
- endocytosis [IMP, ISS]
- intracellular protein transport [ISS]
- membrane organization [TAS]
- metabolic process [ISS, NAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of secretion [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DNA-directed RNA polymerase II, holoenzyme [IDA]
- Golgi apparatus [IDA]
- cell cortex [ISS]
- clathrin-coated endocytic vesicle [IDA]
- cytoplasm [IDA, ISS]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytosol [TAS]
- endocytic vesicle [ISS]
- extracellular vesicular exosome [IDA]
- filamentous actin [IDA, ISS]
- lysosomal membrane [TAS]
- membrane [IDA]
- nuclear membrane [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA, ISS]
- plasma membrane [TAS]
- ruffle [IDA]
- unconventional myosin complex [TAS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 4.96 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID