BAIT
ALDH2
ALDH-E2, ALDHI, ALDM
aldehyde dehydrogenase 2 family (mitochondrial)
GO Process (7)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HSPE1
CPN10, EPF, GROES, HSP10
heat shock 10kDa protein 1
GO Process (3)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Chaperonin GroESL mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli.
An efficient bacterial expression system for the human mitochondrial aldehyde dehydrogenase (ALDH2) was developed using co-overexpression of heat shock chaperone gene GroESL. On the basis of the ALDH2 amino acid sequence and cDNA sequences a full-length cDNA encoding wild-type ALDH2 was cloned from a human liver library. A mutant-type ALDH2 (ALDH2(2)) was developed using site-directed mutagenesis of the ALDH2 cDNA ... [more]
Biochem. Biophys. Res. Commun. Oct. 25, 2002; 298(2);216-24 [Pubmed: 12387818]
Throughput
- Low Throughput
Curated By
- BioGRID