BAIT
TJP2
C9DUPq21.11, DFNA51, DUP9q21.11, PFIC4, X104, ZO2, RP11-16N10.1
tight junction protein 2
GO Process (4)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SORBS1
CAP, FLAF2, R85FL, SH3D5, SH3P12, SORB1, RP11-476E15.1
sorbin and SH3 domain containing 1
GO Process (12)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- cell-matrix adhesion [TAS]
- cellular response to insulin stimulus [ISS]
- focal adhesion assembly [ISS]
- glucose transport [ISS]
- insulin receptor signaling pathway [ISS]
- muscle contraction [TAS]
- positive regulation of establishment of protein localization to plasma membrane [ISS]
- positive regulation of glucose import [ISS]
- positive regulation of glycogen biosynthetic process [ISS]
- positive regulation of lipid biosynthetic process [ISS]
- positive regulation of signal transduction [IC, ISS]
- stress fiber assembly [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 26.67 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID