BAIT
TRAP1
HSP 75, HSP75, HSP90L, TRAP-1
TNF receptor-associated protein 1
GO Process (4)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PNPT1
COXPD13, DFNB70, OLD35, PNPASE, old-35
polyribonucleotide nucleotidyltransferase 1
GO Process (24)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- RNA catabolic process [IDA]
- RNA import into mitochondrion [IDA]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- RNA polyadenylation [IDA]
- cellular response to interferon-beta [IDA]
- cellular response to oxidative stress [IDA]
- mRNA catabolic process [IDA]
- mitochondrial RNA 3'-end processing [IMP]
- mitochondrial RNA 5'-end processing [IMP]
- mitochondrial RNA catabolic process [IDA]
- mitochondrial mRNA catabolic process [IDA]
- mitochondrial mRNA polyadenylation [IMP]
- mitochondrion morphogenesis [ISS]
- mitotic cell cycle arrest [IDA]
- negative regulation of growth [IDA]
- nuclear polyadenylation-dependent mRNA catabolic process [IDA]
- positive regulation of mRNA catabolic process [IMP]
- positive regulation of miRNA catabolic process [IDA]
- positive regulation of mitochondrial RNA catabolic process [IDA]
- protein homooligomerization [IDA]
- protein homotrimerization [IDA]
- rRNA import into mitochondrion [IDA]
- regulation of cellular respiration [ISS]
- regulation of cellular senescence [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 50.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID