BAIT
TUBB
CDCBM6, M40, OK/SW-cl.56, TUBB1, TUBB5, DAAP-285E11.4
tubulin, beta class I
GO Process (8)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DPYSL3
CRMP-4, CRMP4, DRP-3, DRP3, LCRMP, ULIP, ULIP-1
dihydropyrimidinase-like 3
GO Process (10)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- actin crosslink formation [ISS]
- actin filament bundle assembly [ISS]
- axon guidance [TAS]
- cellular response to cytokine stimulus [ISS]
- negative regulation of cell migration [ISS]
- negative regulation of neuron projection development [ISS]
- positive regulation of filopodium assembly [ISS]
- positive regulation of neuron projection development [ISS]
- protein homooligomerization [ISS]
- response to axon injury [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 15.19 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID