RAB27A
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- cellular protein metabolic process [TAS]
- complement-dependent cytotoxicity [IMP]
- exocytosis [IDA]
- exosomal secretion [IMP]
- intracellular protein transport [IBA]
- melanosome localization [IMP]
- melanosome transport [IBA]
- multivesicular body organization [IMP]
- multivesicular body sorting pathway [IMP]
- positive regulation of constitutive secretory pathway [IMP]
- positive regulation of exocytosis [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of phagocytosis [IMP]
- positive regulation of reactive oxygen species biosynthetic process [IMP]
- positive regulation of regulated secretory pathway [IGI, IMP]
- protein secretion [IBA]
- synaptic vesicle transport [TAS]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MYO5A
Gene Ontology Biological Process
- actin filament-based movement [NAS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- membrane organization [TAS]
- post-Golgi vesicle-mediated transport [IMP]
- protein localization to plasma membrane [ISS]
- regulation of Golgi organization [IMP]
- transport [NAS]
- vesicle transport along actin filament [IMP]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [IDA]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [IDA]
- filopodium tip [IDA]
- growth cone [NAS]
- insulin-responsive compartment [ISS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- neuron projection [NAS]
- peroxisome [IDA]
- recycling endosome [IDA]
- ruffle [IDA]
- vesicle [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Melanophilin directly links Rab27a and myosin Va through its distinct coiled-coil regions.
Rab GTPases regulate the membrane transport pathways by recruiting their specific effector proteins. Melanophilin, a putative Rab effector, has recently been identified as a gene that is mutated in leaden mice, in which peripheral localization of melanosomes is impaired in melanocytes. Genetic studies suggest that three coat-color mutation genes, dilute (MyoVa(d)), ashen (Rab27a(ash)), and leaden (Mlph(ln)), act in the same ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MYO5A RAB27A | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID