BAIT
VAPA
VAP-33, VAP-A, VAP33, hVAP-33
VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
GO Process (16)
GO Function (6)
GO Component (8)
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- cell death [IMP]
- endoplasmic reticulum organization [IMP]
- membrane fusion [TAS]
- negative regulation by host of viral genome replication [IDA]
- neuron projection development [IMP]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- protein folding in endoplasmic reticulum [IMP]
- protein localization to endoplasmic reticulum [IMP]
- signal transduction [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HSP90B1
ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1
heat shock protein 90kDa beta (Grp94), member 1
GO Process (13)
GO Function (6)
GO Component (13)
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP]
- actin rod assembly [IDA]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cellular protein metabolic process [TAS]
- cellular response to ATP [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- innate immune response [TAS]
- negative regulation of apoptotic process [IMP, TAS]
- protein transport [NAS]
- regulation of phosphoprotein phosphatase activity [IDA]
- response to hypoxia [IDA]
- sequestering of calcium ion [NAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [IDA]
- endocytic vesicle lumen [TAS]
- endoplasmic reticulum [IDA, TAS]
- endoplasmic reticulum lumen [IDA, TAS]
- endoplasmic reticulum membrane [IDA]
- extracellular matrix [IDA]
- extracellular region [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 30.44 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID