BAIT
VCL
CMD1W, CMH15, HEL114, MV, MVCL, RP11-178G16.3
vinculin
GO Process (15)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- adherens junction assembly [IMP]
- apical junction assembly [IMP]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-matrix adhesion [TAS]
- cellular component movement [TAS]
- epithelial cell-cell adhesion [IMP]
- lamellipodium assembly [ISS]
- morphogenesis of an epithelium [IMP]
- muscle contraction [TAS]
- negative regulation of cell migration [TAS]
- platelet activation [TAS]
- platelet aggregation [IMP]
- platelet degranulation [TAS]
- protein localization to cell surface [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DST
BP240, BPA, BPAG1, CATX-15, CATX15, D6S1101, DMH, DT, EBSB2, HSAN6, MACF2, RP3-361I14.2
dystonin
GO Process (9)
GO Function (5)
GO Component (16)
Gene Ontology Biological Process
- cell motility [IMP]
- cytoskeleton organization [IMP]
- extracellular matrix organization [TAS]
- hemidesmosome assembly [IDA]
- integrin-mediated signaling pathway [NAS]
- intermediate filament cytoskeleton organization [IEP, ISS, NAS]
- maintenance of cell polarity [IMP]
- microtubule cytoskeleton organization [IDA]
- response to wounding [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- actin cytoskeleton [IDA]
- axon [IDA]
- basal plasma membrane [NAS]
- basement membrane [TAS]
- cell leading edge [IDA]
- cytoplasm [IDA, ISS]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- hemidesmosome [IDA, TAS]
- intermediate filament cytoskeleton [IDA]
- microtubule cytoskeleton [IDA]
- microtubule plus-end [IDA]
- nucleus [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 4.61 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID