BAIT
CAV3
caveolin 3
GO Process (56)
GO Function (12)
GO Component (17)
Gene Ontology Biological Process
- actin filament organization [ISO]
- cardiac muscle cell development [ISO]
- caveola assembly [ISO]
- cell differentiation [IEP]
- cell growth [IEP]
- cholesterol homeostasis [ISO]
- cytoplasmic microtubule organization [ISO]
- endocytosis [ISO]
- establishment of protein localization to plasma membrane [ISO]
- glucose homeostasis [ISO]
- heart trabecula formation [ISO]
- membrane raft organization [ISO]
- muscle cell cellular homeostasis [ISO]
- myoblast fusion [ISO]
- myotube differentiation [ISO]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of MAPK cascade [ISO]
- negative regulation of calcium ion transport [ISO]
- negative regulation of cardiac muscle hypertrophy [ISO]
- negative regulation of cell growth involved in cardiac muscle cell development [IMP]
- negative regulation of cell size [ISO]
- negative regulation of nitric-oxide synthase activity [ISO]
- negative regulation of potassium ion transmembrane transport [IMP]
- negative regulation of potassium ion transmembrane transporter activity [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of sarcomere organization [ISO]
- nucleus localization [ISO]
- plasma membrane organization [ISO]
- plasma membrane repair [ISO]
- positive regulation of caveolin-mediated endocytosis [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of microtubule polymerization [IMP]
- positive regulation of myotube differentiation [ISO]
- protein localization [ISO]
- protein localization to plasma membrane [ISO]
- regulation of branching involved in mammary gland duct morphogenesis [ISO]
- regulation of calcium ion import [ISO]
- regulation of calcium ion transmembrane transporter activity [ISO]
- regulation of calcium ion transport [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of heart contraction [IMP]
- regulation of heart rate [ISO]
- regulation of membrane depolarization during cardiac muscle cell action potential [ISO]
- regulation of membrane potential [ISO]
- regulation of nerve growth factor receptor activity [ISO]
- regulation of p38MAPK cascade [ISO]
- regulation of protein kinase B signaling [ISO]
- regulation of signal transduction by receptor internalization [ISO]
- regulation of skeletal muscle contraction [ISO]
- regulation of sodium ion transmembrane transporter activity [ISO]
- regulation of transforming growth factor beta receptor signaling pathway [ISO]
- regulation of ventricular cardiac muscle cell membrane depolarization [ISO]
- regulation of ventricular cardiac muscle cell membrane repolarization [ISO]
- triglyceride metabolic process [ISO]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function- alpha-tubulin binding [ISO]
- calcium channel regulator activity [ISO]
- connexin binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- nitric-oxide synthase binding [IPI]
- potassium channel inhibitor activity [IMP]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [IDA, ISO]
- sodium channel regulator activity [ISO]
- alpha-tubulin binding [ISO]
- calcium channel regulator activity [ISO]
- connexin binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- nitric-oxide synthase binding [IPI]
- potassium channel inhibitor activity [IMP]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [IDA, ISO]
- sodium channel regulator activity [ISO]
Gene Ontology Cellular Component
- T-tubule [IDA, ISO]
- Z disc [IDA]
- caveola [IDA, ISO]
- cell [ISO]
- cell surface [IDA]
- cytoplasm [IDA]
- dystrophin-associated glycoprotein complex [ISO]
- endoplasmic reticulum [ISO]
- integral component of membrane [TAS]
- intercalated disc [IDA]
- membrane [ISO]
- membrane raft [ISO]
- neuromuscular junction [IDA]
- plasma membrane [IDA, ISO]
- protein complex [IDA]
- sarcolemma [IDA, ISO]
- vesicle [IDA]
Rattus norvegicus
PREY
NOS1
bNOS
nitric oxide synthase 1, neuronal
GO Process (53)
GO Function (16)
GO Component (20)
Gene Ontology Biological Process
- aging [IEP]
- arginine catabolic process [IDA]
- behavioral response to cocaine [IMP]
- cellular response to epinephrine stimulus [IMP]
- cellular response to growth factor stimulus [ISO]
- cellular response to mechanical stimulus [IMP]
- exogenous drug catabolic process [ISO]
- female pregnancy [IEP]
- multicellular organismal response to stress [ISO]
- muscle contraction [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of blood pressure [IMP]
- negative regulation of calcium ion transport [ISO]
- negative regulation of cell proliferation [IMP]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of heart contraction [IMP]
- negative regulation of hydrolase activity [ISO]
- negative regulation of insulin secretion [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of potassium ion transport [ISO]
- negative regulation of serotonin uptake [ISO]
- negative regulation of vasoconstriction [IMP]
- nitric oxide biosynthetic process [IDA, ISO]
- nitric oxide mediated signal transduction [IBA, IMP]
- peptidyl-cysteine S-nitrosylation [IDA, ISO]
- positive regulation of adrenergic receptor signaling pathway involved in heart process [ISO]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of histone acetylation [ISO]
- positive regulation of long-term synaptic potentiation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of sodium ion transmembrane transport [IMP]
- positive regulation of the force of heart contraction [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of vasodilation [IMP, ISO]
- regulation of heart contraction [IMP]
- regulation of sensory perception of pain [IMP]
- regulation of sodium ion transport [ISO]
- response to activity [IEP]
- response to estrogen [IDA]
- response to ethanol [IEP]
- response to heat [IEP, ISO]
- response to hypoxia [IEP, ISO]
- response to lead ion [IEP]
- response to lipopolysaccharide [IEP]
- response to nicotine [IEP]
- response to nitric oxide [IEP]
- response to nutrient levels [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IEP]
- response to peptide hormone [IMP]
- response to vitamin E [IEP]
- striated muscle contraction [ISO]
Gene Ontology Molecular Function- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
Gene Ontology Cellular Component
- azurophil granule [IDA]
- cytoplasm [IDA]
- cytoskeleton [ISO]
- cytosol [IDA]
- dendrite [IDA]
- membrane [IDA]
- membrane raft [ISO]
- mitochondrial outer membrane [IDA]
- mitochondrion [IDA]
- nuclear membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- photoreceptor inner segment [IDA]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- protein complex [IDA]
- sarcolemma [IDA, ISO]
- sarcoplasmic reticulum [ISO]
- synapse [IDA, ISO]
- vesicle membrane [IDA]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of neuronal nitric-oxide synthase with caveolin-3 in skeletal muscle. Identification of a novel caveolin scaffolding/inhibitory domain.
Neuronal nitric-oxide synthase (nNOS) has been shown previously to interact with alpha1-syntrophin in the dystrophin complex of skeletal muscle. In the present study, we have examined whether nNOS also interacts with caveolin-3 in skeletal muscle. nNOS and caveolin-3 are coimmunoprecipitated from rat skeletal muscle homogenates by antibodies directed against either of the two proteins. Synthetic peptides corresponding to the membrane-proximal ... [more]
J. Biol. Chem. Nov. 07, 1997; 272(45);28187-90 [Pubmed: 9353265]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NOS1 CAV3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID