BAIT
SNCA
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (70)
GO Function (26)
GO Component (25)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- adult locomotory behavior [ISO]
- aging [IEP]
- behavioral response to cocaine [IMP]
- calcium ion homeostasis [ISO]
- cellular response to copper ion [ISO]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to oxidative stress [ISO]
- dopamine biosynthetic process [ISO]
- dopamine metabolic process [ISO]
- fatty acid metabolic process [ISO]
- long-term synaptic potentiation [ISO]
- membrane organization [ISO]
- microglial cell activation [ISO]
- mitochondrial ATP synthesis coupled electron transport [ISO]
- mitochondrial membrane organization [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of dopamine metabolic process [IMP]
- negative regulation of dopamine uptake involved in synaptic transmission [ISO]
- negative regulation of exocytosis [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of microtubule polymerization [ISO]
- negative regulation of monooxygenase activity [ISO]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of norepinephrine uptake [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of serotonin uptake [ISO]
- negative regulation of thrombin receptor signaling pathway [ISO]
- negative regulation of transporter activity [ISO]
- neutral lipid metabolic process [ISO]
- oxidation-reduction process [ISO]
- phospholipid metabolic process [ISO]
- positive regulation of endocytosis [ISO]
- positive regulation of glutathione peroxidase activity [ISO]
- positive regulation of hydrogen peroxide catabolic process [ISO]
- positive regulation of inositol phosphate biosynthetic process [ISO]
- positive regulation of neurotransmitter secretion [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of receptor recycling [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission [ISO]
- protein destabilization [ISO]
- receptor internalization [ISO]
- regulation of acyl-CoA biosynthetic process [ISO]
- regulation of dopamine secretion [ISO]
- regulation of excitatory postsynaptic membrane potential [ISO]
- regulation of glutamate secretion [ISO]
- regulation of locomotion [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of macrophage activation [ISO]
- regulation of neuron apoptotic process [ISO]
- regulation of neuron death [IGI]
- regulation of neuronal synaptic plasticity [ISO]
- regulation of neurotransmitter secretion [ISO]
- regulation of phospholipase activity [ISO]
- response to cocaine [IEP]
- response to drug [ISO]
- response to interferon-gamma [ISO]
- response to interleukin-1 [ISO]
- response to iron(II) ion [ISO]
- response to lipopolysaccharide [ISO]
- response to magnesium ion [ISO]
- synapse organization [ISO]
- synaptic transmission [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic vesicle endocytosis [ISO]
- synaptic vesicle transport [ISO]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- actin cytoskeleton [ISO]
- axon [ISO]
- axon terminus [IDA]
- cell [ISO]
- cell cortex [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle membrane [IDA]
- cytoskeleton [ISO]
- cytosol [IDA, ISO]
- fibril [ISO]
- growth cone [IDA, ISO]
- inclusion body [ISO]
- intracellular membrane-bounded organelle [IDA]
- mitochondrion [IDA, ISO]
- nuclear outer membrane [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- platelet alpha granule membrane [ISO]
- ribosome [IDA]
- rough endoplasmic reticulum [IDA]
- synapse [ISO]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
Rattus norvegicus
PREY
PRKCE
Pkce
protein kinase C, epsilon
GO Process (32)
GO Function (13)
GO Component (13)
Gene Ontology Biological Process
- TRAM-dependent toll-like receptor 4 signaling pathway [ISO, ISS]
- cellular response to ethanol [ISO]
- cellular response to hypoxia [ISO]
- intracellular signal transduction [IDA]
- lipopolysaccharide-mediated signaling pathway [ISO, ISS]
- locomotory exploration behavior [ISO]
- macrophage activation involved in immune response [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of mitochondrial calcium ion concentration [IMP]
- negative regulation of mitochondrial membrane potential [IMP]
- negative regulation of release of sequestered calcium ion into cytosol [IMP]
- peptidyl-serine phosphorylation [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of actin filament polymerization [ISO, ISS]
- positive regulation of cell-substrate adhesion [ISO]
- positive regulation of cellular glucuronidation [ISO]
- positive regulation of cytokinesis [ISO, ISS]
- positive regulation of epithelial cell migration [ISO, ISS]
- positive regulation of fibroblast migration [ISO, ISS]
- positive regulation of insulin secretion [ISO]
- positive regulation of lipid catabolic process [ISO]
- positive regulation of mucus secretion [ISO]
- positive regulation of receptor activity [ISO]
- positive regulation of synaptic transmission, GABAergic [ISO]
- positive regulation of wound healing [ISO, ISS]
- protein phosphorylation [ISO]
- regulation of insulin secretion involved in cellular response to glucose stimulus [ISO]
- regulation of lipid metabolic process [ISO]
- regulation of peptidyl-tyrosine phosphorylation [ISO]
- release of sequestered calcium ion into cytosol [ISO]
- response to morphine [ISO]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- SH3 domain binding [IPI]
- actin monomer binding [ISO, ISS]
- calcium-independent protein kinase C activity [ISO]
- enzyme activator activity [ISO]
- enzyme binding [ISO]
- ethanol binding [ISO]
- protein binding [IPI]
- protein kinase C activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [ISO]
- receptor activator activity [ISO]
- receptor binding [IPI]
- 14-3-3 protein binding [ISO]
- SH3 domain binding [IPI]
- actin monomer binding [ISO, ISS]
- calcium-independent protein kinase C activity [ISO]
- enzyme activator activity [ISO]
- enzyme binding [ISO]
- ethanol binding [ISO]
- protein binding [IPI]
- protein kinase C activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [ISO]
- receptor activator activity [ISO]
- receptor binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
alpha-Synuclein shares physical and functional homology with 14-3-3 proteins.
alpha-Synuclein has been implicated in the pathophysiology of many neurodegenerative diseases, including Parkinson's disease (PD) and Alzheimer's disease. Mutations in alpha-synuclein cause some cases of familial PD (Polymeropoulos et al., 1997; Kruger et al., 1998). In addition, many neurodegenerative diseases show accumulation of alpha-synuclein in dystrophic neurites and in Lewy bodies (Spillantini et al., 1998). Here, we show that alpha-synuclein ... [more]
J. Neurosci. Jul. 15, 1999; 19(14);5782-91 [Pubmed: 10407019]
Throughput
- Low Throughput
Curated By
- BioGRID