An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Ubiquitin Phosphorylation at Thr12 Modulates the DNA Damage Response.

Walser F, Mulder MPC, Bragantini B, Burger S, Gubser T, Gatti M, Botuyan MV, Villa A, Altmeyer M, Neri D, Ovaa H, Mer G, Penengo L

The ubiquitin system regulates the DNA damage response (DDR) by modifying histone H2A at Lys15 (H2AK15ub) and triggering downstream signaling events. Here, we find that phosphorylation of ubiquitin at Thr12 (pUbT12) controls the DDR by inhibiting the function of 53BP1, a key factor for DNA double-strand break repair by non-homologous end joining (NHEJ). Detectable as a chromatin modification on H2AK15ub, ... [more]

Mol Cell Dec. 05, 2019; 80(3);423-436.e9 [Pubmed: 33022275]

Throughput

Low Throughput

Additional Notes

HIST1H2AB in a nucleosome core particle.
Interaction is dependent on H2A ubiquitination.
Source of TP53BP1 not clear.

Curated By

BioGRID

Interaction Summary

TP53BP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IPI]RNA polymerase II transcription cofactor activity [IMP]methylated histone binding [IDA]p53 binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

HIST1H2AB