BAIT

ERBB2

CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
erb-b2 receptor tyrosine kinase 2
GO Process (30)
GO Function (12)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Blockage of conformational changes of heat shock protein gp96 on cell membrane by a ?-helix peptide inhibits HER2 dimerization and signaling in breast cancer.

Li X, Wang B, Liu W, Gui M, Peng Z, Meng S

Cell membrane translocation of heat shock protein gp96 from the endoplasmic reticulum has been observed in multiple tumors and is associated with tumor malignancy. However, the cancer-intrinsic function and the related mechanism of cell membrane gp96 as a pro-oncogenic chaperone remain further elucidated. In this study, we found that inhibition of gp96 intramolecular conformational changes by a single ?-helix peptide ... [more]

PLoS One Apr. 22, 2015; 10(4);e0124647 [Pubmed: 25898135]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSP90B1 ERBB2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
ERBB2 HSP90B1
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
-

Curated By

  • BioGRID