NFE2L2
Gene Ontology Biological Process
- cellular response to fluid shear stress [IDA]
- cellular response to hydrogen peroxide [IMP]
- cellular response to laminar fluid shear stress [IMP]
- cellular response to tumor necrosis factor [IMP]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of hydrogen peroxide-induced cell death [IGI]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of gene expression [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- proteasomal ubiquitin-independent protein catabolic process [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein ubiquitination [IDA]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
Gene Ontology Cellular Component
DDIT3
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cell redox homeostasis [IDA]
- cellular protein metabolic process [TAS]
- cellular response to DNA damage stimulus [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IDA, IMP, TAS]
- mRNA transcription from RNA polymerase II promoter [IDA]
- negative regulation of canonical Wnt signaling pathway [ISS]
- negative regulation of determination of dorsal identity [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of interleukin-8 production [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- regulation of DNA-templated transcription in response to stress [TAS]
- regulation of transcription involved in anterior/posterior axis specification [ISS]
- regulation of transcription, DNA-templated [IMP]
- response to endoplasmic reticulum stress [IDA]
- response to unfolded protein [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription corepressor activity [TAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription corepressor activity [TAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
- CHOP-C/EBP complex [TAS]
- cytosol [TAS]
- nucleoplasm [IDA, TAS]
- nucleus [IDA, TAS]
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
A functionally defined high-density NRF2 interactome reveals new conditional regulators of ARE transactivation.
NRF2 (NFE2L2) is a cytoprotective transcription factor associated with >60 human diseases, adverse drug reactions and therapeutic resistance. To provide insight into the complex regulation of NRF2 responses, 1962 predicted NRF2-partner interactions were systematically tested to generate an experimentally defined high-density human NRF2 interactome. Verification and conditional stratification of 46 new NRF2 partners was achieved by co-immunoprecipitation and the novel ... [more]
Throughput
- Low Throughput
Additional Notes
- fluorescence cross-correlation spectroscopy
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NFE2L2 DDIT3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NFE2L2 DDIT3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID