BAIT

UBA5

THIFP1, UBE1DC1
ubiquitin-like modifier activating enzyme 5
GO Process (3)
GO Function (2)
GO Component (3)
Homo sapiens

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

UFMylation inhibits the proinflammatory capacity of interferon-?-activated macrophages.

Balce DR, Wang YT, McAllaster MR, Dunlap BF, Orvedahl A, Hykes BL, Droit L, Handley SA, Wilen CB, Doench JG, Orchard RC, Stallings CL, Virgin HW

Macrophages activated with interferon-? (IFN-?) in combination with other proinflammatory stimuli, such as lipopolysaccharide or tumor necrosis factor-? (TNF-?), respond with transcriptional and cellular changes that enhance clearance of intracellular pathogens at the risk of damaging tissues. IFN-? effects must therefore be carefully balanced with inhibitory mechanisms to prevent immunopathology. We performed a genome-wide CRISPR knockout screen in a macrophage ... [more]

Proc Natl Acad Sci U S A Dec. 05, 2020; 118(1); [Pubmed: 33372156]

Throughput

  • Low Throughput

Ontology Terms

  • biological process: response to interferon-gamma (GO:0034341)

Additional Notes

  • Editing Ern1 in Uba5-Cas9-KO cells decreased NO production and restored iNOS protein levels in Uba5-KO cells in response to both IFNgamma and LPS

Curated By

  • BioGRID