BAIT

HCK

JTK9, p59Hck, p61Hck, RP5-836N17.3
HCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (4)
GO Component (6)
Homo sapiens
PREY

IL6ST

CD130, CDW130, GP130, IL-6RB
interleukin 6 signal transducer
GO Process (18)
GO Function (11)
GO Component (8)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Signaling through a novel domain of gp130 mediates cell proliferation and activation of Hck and Erk kinases.

Schaeffer M, Schneiderbauer M, Weidler S, Tavares R, Warmuth M, de Vos G, Hallek M

Interleukin-6 (IL-6) induces the activation of the Src family kinase Hck, which is associated with the IL-6 receptor beta-chain, gp130. Here we describe the identification of an "acidic" domain comprising amino acids 771 to 811 of gp130 as a binding region for Hck, which mediates proliferative signaling. The deletion of this region of gp130 (i.e., in deletion mutant d771-811) resulted ... [more]

Mol. Cell. Biol. Dec. 01, 2001; 21(23);8068-81 [Pubmed: 11689697]

Throughput

  • Low Throughput

Curated By

  • BioGRID